Table S1.
Knockout of Erk1 and Erk2 in mouse ESCs with TALENs and CRISPR/Cas
| Method | Target gene | Cell line | Screened colonies | Uncut, n (%) | One allele cut, n (%) | Two alleles cut, n (%) |
| CRISPR/Cas | Erk1 | V6.5 | 30 | 21 (70.0) | 3 (10.0) | 6 (20.0) |
| iMek1 | 48 | 23 (47.9) | 7 (14.6) | 18 (37.5) | ||
| Erk2−/− | 24 | 21 (87.5) | 3 (12.5) | 0 (0.0) | ||
| Erk2−/−; Erk1+/− | 39 | — | 39 (100.0) | 0 (0.0) | ||
| Erk2 | KH2 | 39 | 28 (71.8) | 4 (10.3) | 7 (17.9) | |
| iMek1 | 48 | 33 (68.8) | 2 (4.2) | 13 (27.0) | ||
| iErk1; Erk1−/− (+Dox) | 45 | 31 (68.9) | 3 (6.7) | 11 (24.4) | ||
| iErk1; Erk1−/− (−Dox) | 24 | 20 (83.3) | 4 (16.7) | 0 (0.0) | ||
| TALENs | Erk1 | iErk1 | 60 | 50 (83.3) | 8 (13.3) | 2 (3.3) |
| iErk2; Erk2−/− (+Dox) | 71 | 64 (90.2) | 3 (4.2) | 4 (5.6) | ||
| Erk2−/− | 48 | 45 (93.8) | 3 (6.2) | 0 (0.0) | ||
| Erk2−/−; Erk1+/− | 41 | — | 41 (100.0) | 0 (0.0) | ||
| Erk2 | V6.5 | 24 | 20 (83.3) | 0 (0.0) | 4 (16.7) |
n, number of ESC clones; %, percentage of ESC clones.