Fig. 2.

Clonal contribution to HSC and adaptive immune cells. Multilineage engraftment is demonstrated by contribution of barcoded clones to HSC, splenic CD3⁺ T cells and CD19⁺ B cells in nine NSG mice that were transplanted with purified barcoded human HSCs. The barcode content of these compartments was determined by comparing the normalized contribution of clones. The relative contributions to the total human HSC (BM) and T- and B-lymphoid lineages (spleen) in each mouse is shown as a ternary plot. Each point represents the contribution of a clone to HSC, CD19⁺ B-cell, and CD3⁺ T-cell lineages. The extent of contribution is depicted by the location of the point along the three axes, with clones contributing equally to all lineages being closer to the center of the triangle. In most mice, but especially in mouse 8 and mouse 9, true multilineage contribution of HSC clones can be observed. The numbers at the points of the triangle indicate the number of detected clones for which contribution to only one lineage was detected.