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The σ70-dependent suboptimal promoter of the E. coli copA gene regulated by the MerR-family metalloregulator CueR. Red arrows, the dyad-symmetric sequence recognized by CueR; gray shades, CueR footprint (18). The RNAP footprint spans from approximately −55 to +29 (10) (SI Appendix, Fig. S1 A and B). Our DNA construct PcopACy3@−5 spans from position −69 to +41; PcopACy3@−41 spans from position −69 to +61.
