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. 2015 Oct 19;112(44):13467–13472. doi: 10.1073/pnas.1515231112

Fig. 4.

Fig. 4.

RNAPCy5−PcopACy3@−5 interactions in the presence of CueR. (A) EFRET histogram with 2 nM [RNAPCy5] in the presence of 200 nM unlabeled holo-CueR. Red lines are resolved peaks at EFRET∼0.07, ∼0.58, and ∼0.87, with percentage areas of 92.0 ± 2.8, 2.9 ± 0.3, and 5.1 ± 0.6%, respectively. (Inset) The corresponding E1/E2 area ratio and that with further adding 1 mM ATP. (B) Same as A, but in the presence of 200 nM unlabeled apo-CueR. The three peaks at EFRET ∼0.07, ∼0.58, and ∼0.87 have percentage areas of 90.8 ± 4.6, 7.4 ± 0.7, and 1.8 ± 0.8%, respectively. (Inset) The corresponding E1/E2 area ratio and that with further adding 1 mM ATP.