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. 2015 Oct 21;112(44):13633–13638. doi: 10.1073/pnas.1512255112

Fig. 3.

Fig. 3.

Characterization of CENH3-interacting sequences. (A) CENH3 ChIP-seq reads mapped against the main RepeatExplorer clusters of the R. pubera genome. Colored circles and names indicate the main centromeric sequences in R. pubera CENH3 ChIP-seq. (B) SIM image showing FISH with CRRh (CL175) and Tyba1+2 on metaphase chromosomes. Arrowheads indicate the longitudinal centromere grooves. (Scale bar: 5 µm.) (C) Annotation of an R. pubera BAC (RpBAC17C8) containing a centromere unit showing a Tyba array of ∼12 kb divided into three subarrays inserted in the protein domains region of a chromovirus-related sequence. Additional transposable elements and single-copy coding sequences were found in close neighborhood.