Fig. 4.

Model of cell-cycle–dependent changes in the holocentromere chromatin organization of R. pubera. During interphase, holocentromeres dissociate into individual CENH3/centromere repeat-containing units. In prophase and metaphase they reassociate and form holocentromeres along the sister chromatids. Most of the CENH3-containing nucleosomes associate with centromere repeat-enriched sequences. Some CENH3-containing nucleosomes associate with centromere repeat-free sequences. Increasing levels of resolution are shown in A, B, and C.