Table S2.
Bacterial strains and plasmids
Bacterial strain or plasmid | Genotype | Reference or source |
E. coli ML35 | Type strain used as prey model | (29, 74) |
E. coli S17-1 | TpR,SmR, recA, thi, pro, hsdR-M+RP4: 2-Tc:Mu: Km, Tn7, λpir | (75) |
E. coli S17-1:: pSSK10:∆pcnB | Used as a donor for conjugating pSSK10:∆pcnB into B. bacteriovorus HD100-S1 | This study |
E. coli S17-1:: pRK-kPR-cI-E-SNA | Used for production of ghosts by over expression of the PhiX174 lysis E gene | This study |
E. coli AT984 | λ−, e14-, dapE6, relA1, spoT1, thiE1; DAP auxotroph | (76, 77) |
E. coli AT984:: pBADsulA-FLAG | Used for production of intact envelopes by osmotic shock | This study |
B. bacteriovorus 109J-S1 | Streptomycin-resistant derivative of the type strain 109J | Laboratory collection |
B. bacteriovorus HD100-S1 | Streptomycin-resistant derivative of the type strain HD100 | (37) |
B. bacteriovorus HD100-S1 ∆pcnB | pcnB mutant | This study |
pSSK10 | oriR6K, mobRP4, cat, sacB, kmR; Suicide vector used for gene knockouts | (65) |
pRK-kPR-cI-E-SNA | Used for production of ghosts by temperature-based induction of the PhiX174 lysis E gene and the Staphylococcal nuclease A gene. | (42) |
pBADsulA-FLAG | pBAD24 harboring sulA CDS, encoding for the division inhibitor SulA, under the ara promoter, | (78) |