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. 2015 Oct 20;112(44):E6028–E6037. doi: 10.1073/pnas.1515749112

Table S2.

Bacterial strains and plasmids

Bacterial strain or plasmid Genotype Reference or source
E. coli ML35 Type strain used as prey model (29, 74)
E. coli S17-1 TpR,SmR, recA, thi, pro, hsdR-M+RP4: 2-Tc:Mu: Km, Tn7, λpir (75)
E. coli S17-1:: pSSK10:∆pcnB Used as a donor for conjugating pSSK10:∆pcnB into B. bacteriovorus HD100-S1 This study
E. coli S17-1:: pRK-kPR-cI-E-SNA Used for production of ghosts by over expression of the PhiX174 lysis E gene This study
E. coli AT984 λ, e14-, dapE6, relA1, spoT1, thiE1; DAP auxotroph (76, 77)
E. coli AT984:: pBADsulA-FLAG Used for production of intact envelopes by osmotic shock This study
B. bacteriovorus 109J-S1 Streptomycin-resistant derivative of the type strain 109J Laboratory collection
B. bacteriovorus HD100-S1 Streptomycin-resistant derivative of the type strain HD100 (37)
B. bacteriovorus HD100-S1 ∆pcnB pcnB mutant This study
pSSK10 oriR6K, mobRP4, cat, sacB, kmR; Suicide vector used for gene knockouts (65)
pRK-kPR-cI-E-SNA Used for production of ghosts by temperature-based induction of the PhiX174 lysis E gene and the Staphylococcal nuclease A gene. (42)
pBADsulA-FLAG pBAD24 harboring sulA CDS, encoding for the division inhibitor SulA, under the ara promoter, (78)