Fig. 3.

RSort-Seq predicts mutations that impair Qrr4 function in E. coli and in V. harveyi. (A) Individual mutations in Qrr4 predicted to have defects examined for repression of luxR-gfp in E. coli. Black bars show fluorescence levels from luxR-gfp in the presence of a vector control (V) and WT Qrr4 (WT). Green bars denote mutations that impair repression. The red bar shows a control base that displays WT repression. The heat map presents results from the RSort-Seq analysis for reference, as in Fig. 2D. Error bars are SDs of at least three trials. (B) As in A for luxO-gfp. (C) The effects of Qrr4 mutations on light production in V. harveyi at OD₆₀₀ = 0.1 are shown. Relative light units (RLU) are counts per minute per milliliter per OD₆₀₀. Error bars are SDs of at least three trials.