Fig. 7.

Double-mutant analysis reveals a suppressor of the Qrr4-U61A defect. (A) Number of reads per bin from the original RSort-Seq analysis for WT Qrr4, Qrr4-A56G, Qrr4-U61A, and Qrr4-A56G, U61A. (B) Fluorescence levels from luxR-gfp in the presence of Qrr4 variants. Black bars show luxR-gfp in the presence of a vector control or WT Qrr4. White bars depict mutant Qrr4 repression of luxR-gfp with and without the secondary mutation Qrr4-A56G. (C) The effect of mutation of Qrr4-A56G on the ability of WT Qrr4 and Qrr4-U61A to regulate light production in V. harveyi at OD₆₀₀ = 0.1. (D) As in B with the designated Qrr4 mutants.