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. 2015 Nov 10;10(11):e0142252. doi: 10.1371/journal.pone.0142252

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© 2015 Okamoto et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — To obtain stable NUSAP1 knockdown transfectants, we performed transfection of the shNUSAP1 and Mock vectors into OSCC cell lines (HSC-3 and Sa3). a,b Expression of NUSAP1 mRNA in shNUSAP1 and Mock cells. c,d Immunoblotting analysis of NUSAP1 protein in shNUSAP1 and Mock cells. The NUSAP1 mRNA and protein are significantly down-regulated in shNUSAP1 cells (HSC-3 and Sa3).