Fig 7. B3C exhibits significantly improved barrier function compared to the transwell model and closely approximates the permeability of neonatal in vivo BBB.
B3C and Transwell BBB were constructed with neonatal RBEC in the presence of ACM. Permeability of 40 kDa dextran in B3C is significantly lower than transwell but not significantly different from that of in vivo BBB in neonatal rats. (** indicates significant difference p<0.01, *** indicates significant difference p<0.001, one-way ANOVA with Tukey’s multiple comparison test; n = 3–4 experiments per group).