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. 2015 Nov 10;10(11):e0142118. doi: 10.1371/journal.pone.0142118

Fig 2. Expression of the Rev1-Vpu fusion protein.

Fig 2

(A) Western blot analysis of HEK293T cells co-transfected with the proviral clones described in Fig 1A or a vpu-deficient mutant thereof. Expression vectors containing rev1-vpu or vpu cassettes served as size controls. Vpu and Rev1-Vpu were detected with an antiserum raised against ZM247 Vpu. (B, C) Expression of Vpu and Rev1-Vpu in ZM247-infected PBMCs or SupD1 cells. Bands representing the Rev1-Vpu fusion protein are highlighted by red arrows. Detection of p55, p24 and actin served as internal controls.