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. 2015 Nov 10;10(11):e0142442. doi: 10.1371/journal.pone.0142442

Fig 4. Genes expression analysis of pTR cells.

Fig 4

(A) RT-PCR analysis of trophoblast and pluripotency genes in pTR cells. The expression of TSC and TR markers such as CDX2, TEAD4, ELF5, HAND1, CDH3, GATA3, EST2, MASH2 and PAG was detected in pPATR-5 and pIVFTR-7, and ESRRB was not detected in both pTR cells but in blastocysts. The pluripotentcy marker OCT4 is only expressed in blastocysts. β-ACTIN gene is used as control. (B) Real-time PCR analysis of trophoblast genes in pTR cells. The genes including CDX2, ELF5 and KRT18 are expressed significantly higher in pPATR-5 than in pIVFTR-7. The gene, HAND1 and GCM1 are expressed significantly higher in pIVFTR-7 than in pPATR-5. (C) Real-time PCR results of imprinted genes in pTR cells. Three paternally imprinted genes, IGF2, PEG1 and PEG10, were expressed lower in pPATR-5 than in pIVFTR-7. (D-E) Real-time PCR results of telomerase activity-related genes in pTR cells. TERC and TERF2 had significantly higher expression in pPATR-5 and pIVFTR-7 than in PFF. β-ACTIN is used as an endogenous control for trophoblasts genes in B) and imprinted genes in C), and GAPDH is for genes, TERC and TERF2. PFF: porcine fetal fibroblast; STO: feeder cells; BL, blastocysts. Error bars indicate ± SEM. The * indicates P < 0.05 and the ** indicates P < 0.001. a,b indicates a statistically significant difference from any another group (P<0.05).