Fig 4. The effect of caffeine and KU55933 on embryonic development after etoposide treatment.

Embryos were treated with etoposide (25 μg/mL) with or without caffeine (5 mM) and/or KU55933 (10 μM). (A) Cleavage and blastocyst rates were detected after 28 h and 7 days of culture, respectively. Data are from 3 independent replicates with a minimum of 20 embryos in each group. * P < 0.05. (B) Apoptosis rates were detected at day 7 of culture. Asterisks denote a significant difference from the controls, which were not treated with etoposide (* p < 0.05). (C) The levels of γH2AX in the nuclei of 1-cell embryos.