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. 2015 Nov 10;10(11):e0142826. doi: 10.1371/journal.pone.0142826

Fig 2. 7TM-mOR- and 6TM-mOR-dependent cAMP regulation.

Fig 2

HEK293 cells transiently transfected with 7TM-, 6TM-mOR and empty vector (control) expressing constructs are subjected to cAMP-sensitive luciferase reporter assay. Increasing concentrations of morphine are applied either to isoproterenol (100 nM, for 5 min) pre-treated cells (A) or to untreated cells (B) to detect inhibition of cAMP production within 30 minutes. Data are analyzed with Prism software (GraphPad, Software, San Diego, CA). Mean ± S.E.M., n = 3 in triplicate.