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. Author manuscript; available in PMC: 2015 Nov 10.
Published in final edited form as: J Immunol. 2014 Oct 22;193(11):5660–5667. doi: 10.4049/jimmunol.1400885

FIGURE 6.

FIGURE 6

Neutrophils lacking DOCK2 and DOCK5 are capable of normal adhesion. BM neutrophils from WT, DOCK2−/−, DOCK5−/−, and DKO mice were incubated on the ICAM-1 or C3bi-coated plates for 30 min at 37°C in the presence or absence of fMLF (10 μM) or PMA (162.12 nM). After being washed twice with HBSS containing 20 mM HEPES (pH 7.4) and 0.1% BSA, the percentage of adherent cells was compared among WT, DOCK2−/−, DOCK5−/−, and DKO neutrophils. Data are indicated as means ± SEM of three independent experiments.