Skip to main content
. Author manuscript; available in PMC: 2015 Nov 10.
Published in final edited form as: J Bone Miner Res. 2011 May;26(5):1099–1110. doi: 10.1002/jbmr.282

Fig. 2.

Fig. 2

Dock5 is necessary for bone resorption by OCs derived from BMMs. (A) OCs were differentiated from Dock5+/+ (+/+) and Dock5−/− (−/−) BMMs and stained for TRACP activity. Scale bars = 200 μm. (B) OCs were differentiated on Osteologic Biocoat from Dock5+/+ and Dock5−/− BMMs and stained for actin to reveal the sealing zones (arrows). Insets show enlarged boxed areas. Scale bars = 100 μm. (C) Proportion of OCs obtained as in panel B with a sealing zone. Graph shows average and SD of three independent OC preparations counting over 300 cells. ap <.001, Mann-Whitney test. (D) Scanning electron micrographs showing OCs differentiated on bone slices. Arrows point at resorption pits. (E) Bone slices from panel D were stained with WGA lectin to reveal resorption pits (arrows). (F) CTX concentrations measured in the culture medium of OCs grown on bone slices as in panels D and E. Graph shows average and SD CTX concentration per OC relative to Dock5+/+ control OC from three independent experiments performed in triplicate. ap <.001 Mann-Whitney test.