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. Author manuscript; available in PMC: 2015 Nov 10.
Published in final edited form as: J Bone Miner Res. 2011 May;26(5):1099–1110. doi: 10.1002/jbmr.282

Fig. 3.

Fig. 3

Dock5 is dispensable for the expression of OC characteristic genes and for Rac1 activation in response to M-CSF and adhesion. (A, B) Total RNAs were prepared from Dock5+/+ (+/+) and Dock5−/− (−/−) BMMs grown for 5 days in the presence of M-CSF (black bars) or RANKL and M-CSF (white bars). mRNAs levels of OC characteristic genes (A) and from Dock1 and Dock2 (B) relative to Gapdh were determined by RT-PCR. (C) Rac1 activity was measured by G-LISA in Dock5+/+ and Dock5−/− OCs on day 4 of differentiation stimulated with 100 ng/mL of M-CSF for the indicated amount of time. (D) The levels of total and phosphorylated Erk1/2 were determined by Western blot in Dock5+/+ and Dock5−/− OCs stimulated with M-CSF as in panel C. (E) Rac1 activity was measured by G-LISA in Dock5+/+ and Dock5−/− OCs on day 4 of differentiation lifted and left in suspension (s) or replated onto vitronectin-coated plates for 30 minutes (a). Panels C and E show average active Rac1 and SD of duplicate measures in one experiment representative of two independent OC preparations.