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. Author manuscript; available in PMC: 2015 Nov 10.
Published in final edited form as: J Bone Miner Res. 2011 May;26(5):1099–1110. doi: 10.1002/jbmr.282

Fig. 6.

Fig. 6

Suppression of Dock5 leads to reduced trabecular bone mass in mice. (A) Levels of Dock5 mRNA relative to Gapdh mRNA determined by RT-PCR in BMMs grown with M-CSF (ND) or differentiated into OCs for 5 days (D5) and in proliferating MSCs and MSC-derived osteoblasts (OB). (B) mRNA levels of the indicated osteoblast differentiation marker genes relative to Gapdh in proliferating MSCs and osteoblasts from panel A and mineralization activity of the osteoblasts revealed by alizarin red S staining. (C) Total proteins extracted from BMMs grown with M-CSF (ND) or for 3 and 5 days with M-CSF and RANKL and from various mouse tissues (Ey = eye; Sp = spleen; St = stomach; Te = testis; Pl = placenta; Lu = lung; Br = brain; He = heart; Li = liver; Ki = kidney; Mu = muscle) were analyzed by Western blot with antibodies against Dock5 and tubulin for normalization. (D) Percent bone volume/total volume (BV/TV) in distal femurs of 7-week-old mice (n = 6). ap <.001, Mann-Whitney test. (E) Histologic section showing distal femur of 7-week-old mouse stained with von Kossa to reveal mineralized tissue. (F) OC number per bone perimeter (OC/mm) in distal femurs of 7-week-old mice (n = 6). (G) Histologic sections from distal femurs of 7-week-old mice stained for TRACP activity to visualize OCs and counterstained with hematoxylin to visualize bone trabeculae.