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. Author manuscript; available in PMC: 2016 Nov 14.
Published in final edited form as: FEBS Lett. 2015 Aug 20;589(22):3419–3432. doi: 10.1016/j.febslet.2015.08.015

Figure 2.

Figure 2

A) Reaction mechanism for nitrocefin hydrolysis by di-Zn(II) B1 and B3 enzymes. EI is the experimentally characterized anionic intermediate interacting with Zn2 via the N atom and the carboxylate [66,91]. The same scheme would be valid for other chromogenic cephalosporins such as cromaceph and CENTA [92]. B) Reaction mechanism for imipenem hydrolysis by di-Zn(II) B1 and B3 enzymes. EI1 and EI2 are the experimentally characterized anionic intermediates [23], stabilized by interaction with Zn2. The representation of EI2 is based on the crystallographic structures of NDM-1 in complex with hydrolyzed meropenem (PDB 4EYL, see Figure 4) [44]. W1 stands for Wat1 and W2 stands for Wat2.