Table 1.
Primers used for qPCR of day 7 fracture callus
| Gene of Interest | Abbreviation | Associated Process | Oligo Sequence or Primer Set |
|---|---|---|---|
| Aggrecan | Acan | Chondrogenesis | Mm.PT.58.23585796 |
| Bone Morphogenetic Protein 2 (exon 3) | Bmp2 | Osteo- & Chondrogenesis | F: TGG AAG TGG CCC ATT TAG AG, R: TGA CGC TTT TCT CGT TTG TG |
| Bone Morphogenetic Protein 3 (exons 1–2) | Bmp3 | Osteogenesis Antagonist | Mm.PT.58.8861659 |
| Bone Morphogenetic Protein 4 (exons 1–2) | Bmp4 | Osteogenesis | Mm.PT.58.7116776 |
| Bone Morphogenetic Protein 7 (exons 2–3) | Bmp7 | Osteogenesis | Mm.PT.58.30464128 |
| Bone Sialoprotein | Bsp | Osteogenesis | F: CCG GCC ACG CTA CTT TCT T, R: GGA CTG GAA ACC GTT TC |
| Chordin (exons 21–23) | Chrd | BMP Antagonist | Mm.PT.58.30333725 |
| Collagen type 1a1 | Col1 | Osteogenesis | F: GCTCCT CTT AGG GGC CAC T, R: CCA CGT CTC ACC ATT GGG G |
| Collagen type 2a1 (exons 48–50) | Col2 | Chondrogenesis | Mm.PT.58.28573530 |
| Gremlin1 (exons 1–2) | Grem1 | BMP Antagonist | Mm.PT.58.11631114 |
| Noggin (exon 1) | Nog | BMP Antagonist | Mm.PT.58.30936710.g |
| Osterix | Osx | Osteogenesis | F: CCC TTC TCA AGC ACC AAT GG, R: AAG GGT GGG TAG TCA TTT GCA TA |
| Sox9 | Sox9 | Osteo- & Chondrogenesis | F : GAG CCG GAT CTG AAG AGG GA, R: GCT TGA CGT GTG GCT TGT TC |
| Vascular Endothelial Growth Factor | Vegf | Angiogenesis | Mm.PT.58.31754187 |
| Importin 8 (exons 7–9) | Ipo8 | Reference Gene | Mm.PT.58.6409954 |
| TATA Binding Protein (exons 2–3) | Tbp | Reference Gene | Mm.PT.58.42394711 |