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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Bone. 2015 Jul 17;81:168–177. doi: 10.1016/j.bone.2015.07.018

Fig. 4. SIRT6 interacts physically with Runx2/Osx and is recruited to their target promoters.

Fig. 4

(A) Western blots showing expression of SIRT6, Runx2, and Osx in NIH3T3 cells with retroviral infection of SIRT6 and transient transfection of Runx2 or Osx. αTubulin was used as a loading control. (B) SIRT6 binding to Runx2 and Osx, as determined by western blots of SIRT6 or Flag antibody immunoprecipitates from SIRT6 WT overexpressing cells with Flag-Runx2 or Flag-Osx. IgG antibody was used as a negative control for IP assay. The arrow indicates Runx2 and Osx expression. (C) ChiP analysis to detect SIRT6 at the indicated promoters in SIRT6 overexpressing MC3T3-E1 cells with or without BMP-2 treatment. Data represented means ± S.E. of three experiments in triplicate. *, p < 0.01. (D). ChiP analysis to detect endogenous SIRT6 at the indicated promoters in osteoblasts with BMP-2 stimulation (24h).