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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Neurobiol Dis. 2015 Jun 6;82:152–163. doi: 10.1016/j.nbd.2015.05.020

Figure 5. Pre-incubation of scyllo-inositol with soluble Aβ oligomers prevents their binding to neurons and synaptotoxic effects.

Figure 5

Representative blots showing eluted A β in culture medium (A) and bound on membrane (B) of hippocampal neurons (DIV18) under different treatments. (C) Confocal images show surface-labeled Aβ (detected by 3D6, green), and total VGLUT2 (red) of hippocampal neurons (DIV18) after 12 hr treatment with eluted wt Aβ40 or (Aβ40 S26C)2 dimer, with or without co-administration of chiro-or scyllo-inositol (20 μM). Scale bar, 20 μm. (D) Confocal images showing endogenous PSD95 (green) and synapsin I (SYN-I, red) in hippocampal neurons (DIV18) after 1 d treatment with eluted synthetic wt Aβ40 or (Aβ40 S26C)2 dimer, with or without co-administration of chiro-or scyllo-inositol (20 μM). Scale bar, 20 μm.