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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Invest Dermatol. 2015 Jun 22;135(11):2894–2897. doi: 10.1038/jid.2015.235

Figure 2. Dact1 attenuates Wnt and Tgfβ signaling in 3T3-J2 cells.

Figure 2

(a and b) Enhanced Tgfβ2 mRNA (a) and protein (b) levels in Dact1-silenced 3T3-J2 cells at day 4 by Wnt3A. (c) Reduced Igf2 expression by loss of Dact1 and further suppression by Tgfβ2 treatment in Dact1-silenced 3T3-J2 cells. (d) Decreased basal Igf2 protein levels in Dact1-silenced 3T3-J2 cells. (e and f) Enhanced Smad2/3 phosphorylation in Dact1-silenced 3T3-J2 cells. (g) Recovery of Igf2 expression by RepSox in Dact1-silenced 3T3-J2 cells. (h) Model for the role of Dact1. Data shown are mean±SEM (n=3). *P<0.05; **P<0.01.