(A) Periosteal mineral apposition rate (PsMAR), mineralizating surface (PsMS/BS), and bone formation rate (PsBFR/BS) were measured in unstained sections from femoral midshaft at 4.5 months of age. (B) Representative images show green and red fluorochrome labels. (C) Endosteal mineral apposition rate (EcMAR), mineralizating surface (EcMS/BS), and bone formation rate (EcBFR/BS) were measured in the same bone section as (A). Bars represent mean ± s.d., n=7/group. *p<0.05 versus Cx43fl/fl mice, and #p<0.05 versus corresponding vehicle-treated mice, by two-way ANOVA.