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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Invest Dermatol. 2015 Jun 3;135(11):2775–2784. doi: 10.1038/jid.2015.204

Figure 5. IFN-γ stimulation augments MIF-CD74 signaling.

Figure 5

Cells were treated with ISO-1 for 24 hours (c and d) or 48 hours (a, b and e) under IFN-γ–stimulatory conditions. AKT Ser473 phosphorylation (a) and BCL-2 protein expression (b) were detected by Western blot analysis. qRT-PCR was performed to measure mRNA of BCL-2 (c), IL-6 and IL-8 (d). *P < 0.05. (e) Cell culture supernates were subjected to focused cytokine array. Dots square, positive control; #1, IFN-γ; #2, IL-6; #3, IL-8; #4, MIF; a, CXCL1. Lower bar graph indicates relative pixel densities of IL-6 and IL-8.