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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: J Mol Cell Cardiol. 2015 Sep 24;88:101–110. doi: 10.1016/j.yjmcc.2015.09.010

Fig.2. Isoproterenol induced expression of MITF and BRG1 in H9c2 cells.

Fig.2

(A) RNA was harvested from untreated H9c2 cells (con) and H9c2 cells that were treated with 10μM isoproterenol (Iso) for 4 hours. Relative MITF, BRG1, BNP, ANP expression levels were measured by quantitative RT- PCR (qRT – PCR) and normalized to 18S rRNA. (B) Cells treated as in A were harvested 24 hours after isoproterenol stimulation. RT-qPCR was performed as in A. Standard error bars and statistical significance are shown (*p<0.05, ** p<0.01 and *** p<0.005). (C) Whole cell extracts were prepared from H9C2 cells treated as in (A), run on an SDS-PAGE gel, and immunoblotted with anitbodies to MITF and BRG1. Tubulin is a loading control. The blots shown are from three independent experiments. Band densities were quantified using Image J software and shown in the graphs below each blot.