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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: J Invest Dermatol. 2015 Jul 2;135(11):2611–2622. doi: 10.1038/jid.2015.248

Figure 2. Switching on in situ ROS production in the skin activates stem cell proliferation in the hair follicle niche and promotes a transient proliferation of epidermal and dermal cells.

Figure 2

a) BrdU label retaining cells (LRC) quantification in the hair follicle bulge region. The mean + SE (n=4) is represented. b) Immunological detection of the Ki67 proliferation marker in the hair follicle bulge region. c) Dorsal skin histological sections stained for inmunohistochemical detection of Ki67 (left panels) or with Masson′s trichrome (middle panels) showing transient effects in the skin induced by mALA+Light treatments, including cell proliferation, hyperplasia in the epidermis (vertical bars), a significant increase in dermal cellularity (squares) and strong cornification (arrowheads). Squares indicate equivalent areas in which cell numbers were quantified. Right panels show the quantification of interfollicular epidermis (IFE) thickness and dermal cellularity in 10 histological fields. The mean + SD (n=3) is represented. In a) and b) representative confocal microscopy images (maximum projections) of tail skin whole-mounts are shown. Bars: 100 μm.