Table 1.
Proteins identified as enriched in broth supernatant compared to CP/PP fraction
| Name | Accession | Enrichment (all time points)a |
Enrichment (significant time points)b |
Relative abundance in membranec |
|---|---|---|---|---|
| Constitutived | ||||
| serine protease (htrA) | HP1019h | 5.08 | 5.99 | 98.48% |
| protease (pqqE) | HP1012 | 4.68 | 4.68 | 89.39% |
| colicin tolerance-like protein (tolB) | HP1126 | 4.19 | 4.60 | 91.59% |
| conserved hypothetical secreted protein | HP0785 | 3.96 | 3.96 | 37.30% |
| catalase-like protein | HP0485 | 3.74 | 3.74 | 73.21% |
| putative neuraminyllactose-binding hemagglutinin homolog (hpaA) | HP0410h | 3.06 | 3.68 | 81.19% |
| 2’,3’-cyclic-nucleotide 2’-phosphodiesterase (cpdB) | HP0104h | 3.65 | 3.65 | 72.15% |
| membrane-associated lipoprotein (lpp20) | HP1456h | 2.72 | 3.60 | 87.45% |
| processing protease (ymxG) | HP0657 | 3.12 | 3.12 | 76.45% |
| hypothetical protein | HP0408h | 2.73 | 3.07 | 64.10% |
| dipeptide ABC transporter, periplasmic dipeptide- binding protein (dppA) | HP0298 | 3.05 | 3.05 | 42.82% |
| membrane bound endonuclease (nuc) | HP0323 | 3.01 | 3.01 | 79.76% |
| conserved hypothetical secreted protein | HP0235 | 2.74 | 3.00 | 36.32% |
| hypothetical protein | HP0304 | 2.90 | 2.90 | 26.62% |
| conserved hypothetical protein | HP1285h | 2.85 | 2.85 | 67.70% |
| hypothetical protein | HP1173h | 2.71 | 2.71 | 61.34% |
| hypothetical protein | HP1454 | 2.64 | 2.64 | 57.61% |
| gamma-glutamyltranspeptidase (ggt) | HP1118 | 2.43 | 2.43 | 69.40% |
| thiol:disulfide interchange protein (dsbC), putative | HP0377h | 2.39 | 2.39 | 45.95% |
| conserved hypothetical secreted protein | HP0211 | 2.32 | 2.37 | 27.73% |
| hypothetical protein | HP0953 | 2.08 | 2.08 | 24.06% |
| conserved hypothetical secreted protein | HP1098 | 2.06 | 2.06 | 41.83% |
| iron(III) ABC transporter, periplasmic iron-binding protein (ceuE) | HP1562 | 2.01 | 2.01 | 51.38% |
| catalase | HP0875h | 1.77 | 1.98 | 64.17% |
| outer membrane protein | HP1564h | 1.77 | 1.77 | 21.53% |
| Earlye | ||||
| protective surface antigen D15 | HP0655h | 1.89 | 4.74 | 95.85% |
| amino acid ABC transporter, periplasmic binding protein (yckK) | HP0940 | 3.46 | 4.29 | 40.06% |
| flagellar sheath adhesin hpaA | HP0797h | 1.35 | 3.23 | 93.01% |
| outer membrane protein (omp9) | HP0317h | 0.27 | 3.10 | 94.79% |
| hypothetical protein | HP0688 | 1.59 | 3.03 | 14.97% |
| conserved hypothetical protein | HP0507 | 2.19 | 2.89 | 24.74% |
| peptide deformylase (def) | HP0793 | 1.85 | 2.76 | 13.55% |
| purine-nucleoside phosphorylase (deoD) | HP1178 | 1.82 | 2.65 | 11.68% |
| hypothetical protein | HP1265 | 1.67 | 2.61 | 11.92% |
| conserved hypothetical protein | HP0233 | 1.46 | 2.36 | 17.80% |
| phosphoglycerate mutase (pgm) | HP0974 | 1.18 | 2.03 | 12.09% |
| triosephosphate isomerase (tpi) | HP0194 | 1.35 | 1.95 | 8.22% |
| pantoate-beta-alanine ligase (panC) | HP0006 | 1.35 | 1.91 | 4.88% |
| serine hydroxymethyltransferase (glyA) | HP0183 | 1.41 | 1.88 | 28.91% |
| octaprenyl-diphosphate synthase (ispB) | HP0240 | 1.23 | 1.82 | 15.53% |
| hypothetical protein | HP0218 | 1.12 | 1.75 | 20.78% |
| NAD(P)H-flavin oxidoreductase | HP0642 | 1.20 | 1.59 | 6.79% |
| Variedf | ||||
| hypothetical protein | HP1457 | 3.83 | 5.70 | 95.91% |
| outer membrane protein (omp28) | HP1243h | 2.81 | 5.58 | 98.09% |
| outer membrane protein (omp11) | HP0472 | 3.45 | 5.12 | 92.02% |
| outer membrane protein (omp19) | HP0896h | 1.74 | 4.96 | 97.89% |
| conserved hypothetical protein | HP0710h | 3.66 | 4.94 | 97.52% |
| outer membrane protein (omp6) | HP0229h | 2.84 | 4.75 | 95.90% |
| hypothetical protein | HP1455 | 3.99 | 4.59 | 47.31% |
| (3R)-hydroxymyristoyl-(acyl carrier protein) dehydratase (fabZ) | HP1376 | 2.41 | 4.41 | 79.40% |
| CDP-diglyceride hydrolase (cdh) | HP0871 | 2.70 | 3.33 | 40.26% |
| aspartate-semialdehyde dehydrogenase (asd) | HP1189 | 2.10 | 3.30 | 49.80% |
| hypothetical protein | HP0783 | 2.44 | 2.84 | 13.97% |
| outer membrane protein (omp27) | HP1177h | 0.36 | 2.53 | 89.56% |
| hypothetical protein | HP0973h | 1.82 | 2.41 | 47.86% |
| xanthine guanine phosphoribosyl transferase (gpt) | HP0735 | 1.31 | 2.07 | 6.72% |
| arginyl-tRNA synthetase (argS) | HP0319 | 1.49 | 1.93 | 4.37% |
| Lateg | ||||
| ATP-dependent nuclease (addB) | HP0275h | 3.74 | 4.71 | 91.01% |
| outer membrane protein (omp30) | HP1395 | 2.56 | 4.58 | 90.75% |
| methionine amino peptidase (map) | HP1299 | 2.38 | 4.29 | 49.54% |
| membrane fusion protein (mtrC) | HP0606h | 3.81 | 3.62 | 93.81% |
| soluble lytic murein transglycosylase (slt) | HP0645h | 3.10 | 3.15 | 77.55% |
| L-asparaginase II (ansB) | HP0723h | 2.00 | 2.71 | 26.44% |
| hypothetical protein | HP0721 | 1.67 | 2.66 | 46.54% |
| cag pathogenicity island protein (cag24) | HP0545 | 1.18 | 2.54 | 17.02% |
| carbonic anhydrase | HP1186 | 1.99 | 2.36 | 62.21% |
| hypothetical protein | HP0129 | 1.19 | 2.34 | 30.36% |
| conserved hypothetical secreted protein | HP1286 | 1.29 | 2.29 | 22.54% |
| cytochrome c553 | HP1227 | 1.14 | 2.27 | 11.75% |
| iron(III) ABC transporter, periplasmic iron-binding protein (ceuE) | HP1561 | 1.67 | 2.01 | 49.09% |
| hypothetical protein | HP0231h | 1.61 | 2.00 | 54.73% |
| vacuolating cytotoxin | HP0887 | −0.39 | 2.00 | 89.98% |
| hypothetical protein | HP0204 | 1.58 | 1.92 | 24.75% |
| outer membrane protein (omp2) | HP0025h | 1.08 | 1.72 | 83.40% |
a
Relative abundance in the supernatant compared to the CP/PP fraction (Esup): [Esup=log2(%abundancesupernatant/% abundanceCP/PP)]. Mean Esup values were calculated based on analysis of all five time points.
b
Average of significant enrichment (Esup) values.
c
Relative % abundance in the membrane fraction compared to CP/PP fraction: [%abundancemembrane/(%abundancemembrane+%abundanceCP/PP)]. Mean % abundance values were calculated based on analysis of all five time points.
d
Enriched at 4 or all 5 time points.
e
Enriched at two of the three earlier time points (12, 24, or 30h).
f
Enriched at varied time points with no clear pattern.
g
Enriched at two of the three later time points (30, 36, or 48h).
h
Previously detected on the bacterial surface by a biotinylation-based method (reference 17).