Table 2.
Selectively released H. pylori supernatant proteins identified in previous studies and confirmed in current study.
| Name | Accession | Methodd | Phase of enrichment |
Reference | Predicted cleavage sitee |
|---|---|---|---|---|---|
| hypothetical protein | HP0129 | 1D | late | b | 21 |
| outer membrane protein (omp6) | HP0229 | 1D | varied | c | - |
| hypothetical protein | HP0231 | 1D | late | a,b | - |
| ATP-dependent nuclease (addB) | HP0275 | both | late | c | 22 |
| dipeptide ABC transporter substrate-binding protein DppA | HP0298 | 1D | constitutive | c | − |
| thiol:disulfide interchange protein (dsbC), putative | HP0377 | both | constitutive | a | 24 |
| putative neuraminyllactose-binding hemagglutinin homolog (hpaA) | HP0410 | both | constitutive | c | 24 |
| outer membrane protein (omp11) | HP0472 | 1D | varied | c | 18 |
| Cag pathogenicity island protein (cag24) | HP0545 | both | late | c | 30 |
| processing protease (ymxG) | HP0657 | both | constitutive | c | 20 |
| conserved hypothetical protein | HP0710 | 1D | varied | c | - |
| hypothetical protein | HP0721 | 1D | late | b | 20 |
| catalase | HP0875 | both | constitutive | c | - |
| vacuolating cytotoxin | HP0887 | both | late | a,b,c | 33 |
| hypothetical protein | HP0953 | both | constitutive | c | 21 |
| hypothetical protein | HP0973 | 1D | varied | b | 28 |
| protease (pqqE) | HP1012 | both | constitutive | c | 28 |
| serine protease (htrA) | HP1019 | both | constitutive | a,c | - |
| conserved hypothetical secreted protein | HP1098 | both | constitutive | a,c | 25 |
| gamma-glutamyltranspeptidase (ggt) | HP1118 | both | constitutive | a,c | 26 |
| hypothetical protein | HP1173 | both | constitutive | a,c | 26 |
| carbonic anhydrase | HP1186 | 1D | late | a,c | 18 |
| conserved hypothetical protein | HP1285 | both | constitutive | c | - |
| conserved hypothetical secreted protein | HP1286 | both | late | a,b,c | 17 |
| outer membrane protein (omp30) | HP1395 | 1D | late | c | 21 |
| hypothetical protein | HP1454 | both | constitutive | a,c | 18 |
| membrane-associated lipoprotein (lpp20) | HP1456 | both | constitutive | c | - |
| hypothetical protein | HP1457 | 1D | varied | c | 28 |
| outer membrane protein | HP1564 | both | constitutive | b | 28 |
(a)
Bumann et al. (reference 8)
(b)
Kim et al., (reference 9)
(c)
Smith et al., (reference 10)
d
Method indicates the mass spectrometry technique used to identify the protein in the current study. Proteins that were identified in MudPIT and 1D experiments are labeled “both”.
e
Predicted signal sequence cleavage site. Numbers represent the position of the amino acid N-terminal to the site of predicted cleavage. Dash (−) signifies that no signal sequence was predicted.