Table 3.
Selectively released H. pylori supernatant proteins identified in the current study but not previous studies.
| Name | Accession | Methoda | Phase of enrichment |
Predicted cleavage siteb |
|---|---|---|---|---|
| pantoate-beta-alanine ligase (panC) | HP0006 | 1D | early | - |
| outer membrane protein (omp2) | HP0025 | 1D | late | 20 |
| 2’,3’-cyclic-nucleotide 2’-phosphodiesterase (cpdB) | HP0104 | both | constitutive | - |
| translation elongation factor EF-P (efp) | HP0177 | MudPIT | constitutive | - |
| serine hydroxymethyltransferase (glyA) | HP0183 | 1D | early | - |
| triosephosphate isomerase (tpi) | HP0194 | both | early | - |
| hypothetical protein | HP0204 | both | late | 19 |
| conserved hypothetical secreted protein | HP0211 | 1D | constitutive | - |
| hypothetical protein | HP0218 | 1D | early | - |
| conserved hypothetical protein | HP0233 | 1D | early | - |
| conserved hypothetical secreted protein | HP0235 | both | constitutive | - |
| octaprenyl-diphosphate synthase (ispB) | HP0240 | 1D | early | - |
| hypothetical protein | HP0304 | 1D | constitutive | 19 |
| outer membrane protein (omp9) | HP0317 | 1D | early | 20 |
| arginyl-tRNA synthetase (argS) | HP0319 | both | varied | - |
| membrane bound endonuclease (nuc) | HP0323 | 1D | constitutive | - |
| hypothetical protein | HP0408 | 1D | constitutive | - |
| catalase-like protein | HP0485 | both | constitutive | 24 |
| conserved hypothetical protein | HP0507 | 1D | early | - |
| membrane fusion protein (mtrC) | HP0606 | 1D | late | - |
| adenylate kinase (adk) | HP0618 | MudPIT | constitutive | - |
| NAD(P)H-flavin oxidoreductase | HP0642 | 1D | early | - |
| soluble lytic murein transglycosylase (slt) | HP0645 | 1D | late | - |
| protective surface antigen D15 | HP0655 | 1D | early | - |
| hypothetical protein | HP0688 | 1D | early | - |
| L-asparaginase II (ansB) | HP0723 | both | late | - |
| xanthine guanine phosphoribosyl transferase (gpt) | HP0735 | 1D | varied | - |
| hypothetical protein | HP0783 | 1D | varied | - |
| conserved hypothetical secreted protein | HP0785 | 1D | constitutive | 19 |
| peptide deformylase (def) | HP0793 | 1D | early | - |
| flagellar sheath adhesin hpaA | HP0797 | 1D | early | - |
| CDP-diglyceride hydrolase (cdh) | HP0871 | 1D | varied | 21 |
| outer membrane protein (omp19) | HP0896 | 1D | varied | 19 |
| amino-acid ABC transporter, periplasmic binding protein (yckK) | HP0940 | both | early | - |
| phosphoglycerate mutase (pgm) | HP0974 | 1D | early | - |
| colicin tolerance-like protein (tolB) | HP1126 | both | constitutive | - |
| outer membrane protein (omp27) | HP1177 | 1D | varied | 21 |
| purine-nucleoside phosphorylase (deoD) | HP1178 | 1D | early | - |
| aspartate-semialdehyde dehydrogenase (asd) | HP1189 | 1D | varied | - |
| cytochrome c553 | HP1227 | 1D | late | 19 |
| outer membrane protein (omp28) | HP1243 | 1D | varied | 20 |
| hypothetical protein | HP1265 | 1D | early | - |
| methionine amino- peptidase (map) | HP1299 | 1D | late | - |
| (3R)-hydroxymyristoyl-(acyl carrier protein) dehydratase (fabZ) | HP1376 | 1D | varied | - |
| hypothetical protein | HP1455 | 1D | varied | - |
| iron(III) ABC transporter, periplasmic iron-binding protein (ceuE) | HP1561 | both | late | 31 |
| iron(III) ABC transporter, periplasmic iron-binding protein (ceuE) | HP1562 | both | constitutive | 29 |
a
Method indicates the mass spectrometry technique used to identify the protein in the current study. Proteins that were identified in MudPIT and 1D experiments are labeled “both”.
b
Predicted signal sequence cleavage site. Numbers represent the position of the amino acid N-terminal to the site of predicted cleavage. Dash (−) signifies that no signal sequence was predicted.