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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Neurobiol Dis. 2015 Jun 6;82:164–175. doi: 10.1016/j.nbd.2015.05.016

Figure 8. mIPSC peak amplitudes and current kinetic timecourse are altered in Hetα1KO and Hetα1AD cortex.

Figure 8

A) Sample mIPSC traces obtained from P35 (left) and P120 (right) wild type, Hetα1KO, and Hetα1AD layer II/III pyramidal neurons in the motor cortex. B) Compared to wild type (−31.9 ± 3.0 pA), the magnitude of peak mIPSC amplitude was decreased in Hetα1KO (−23.8 ± 2.1pA, P = 0.037) and Hetα1AD cortex (−16.3 ± 1.3 pA, P < 0.001). The difference in mIPSC amplitude between Hetα1KO and Hetα1AD was statistically significant (P = 0.034). The magnitude of mIPSC peak current amplitudes increased from P35 (−21.0 ± 1.6 pA) to P120 (−26.5 ± 2.4 pA, P = 0.030) without any significant interaction between genotype and age. C) The mIPSC 10-90% rise times were greater in Hetα1KO (2.0 ± 0.2 ms, P = 0.010 vs. wild type), and Hetα1AD (3.1 ± 0.8 ms, P < 0.001 vs. wild type) than wild type neurons (1.3 ± 0.1 ms). The differences in rise times between Hetα1KO and Hetα1AD neurons (P = 0.677) and P35 (2.0 ± 0.2 ms) and P120 (1.7 ± 0.1 ms, # P = 0.079) mIPSCs were not statistically significant. D) Compared with wild type neurons (7.9 ± 0.8 ms), the decay τ, was increased in Hetα1KO (12.6 ± 1.1 ms, P = 0.021) and Hetα1AD (13.1 ± 1.4 ms, P = 0.007).There was no significant difference between the values of decay τ at P35 (11.7 ± 1.1 ms) and P120 (10.8 ± 1.0 ms, P = 0.492). Sample sizes (neurons recorded) were N = 18 wild type, 18 Hetα1KO, 19 Hetα1AD, 26 P35, and 29 P120. * = P < 0.05, ** = P < 0.01, *** = P < 0.001, ns = nonsignificant.