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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: Behav Brain Res. 2015 Jun 11;295:35–44. doi: 10.1016/j.bbr.2015.06.013

Figure 7.

Figure 7

Treatment with 5,7-DHT (grey bars) significantly reduced TPH2 cell numbers in several DRN sub-regions. EtOH-consuming rats treated with 5,7-DHT (panel B, grey bars) showed significantly more TPH2 cell depletion across all VM sub-regions compared to all other treatment groups, including non-drinkers experiencing the same treatment (panel A; # = p < .012). Within drinkers (B), 5,7-DHT treated animals had a significant decrease in TPH2 cell numbers compared to vehicle treated animals across all sub-regions except cLAT and rLAT (* = p < .05) It is important to note that the rostral and caudal “lateral wings” are not true sub-regions of the DRN (Vasudeva and Waterhouse, 2014). These areas were quantified as controls for negative TPH2 staining and to ensure proper identification of other DRN sub-regions.Interestingly, there was no difference in depletion in all VM sub-regions of non-drinkers, regardless of treatment, and this sub-region was preserved in Drinkers (B). Data are represented by mean ± SEM.