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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Neurobiol Dis. 2015 Sep 1;82:574–579. doi: 10.1016/j.nbd.2015.08.022

Figure 2. Urate induces the Nrf2 pathway in astrocytes.

Figure 2

(A) GSH levels are elevated in lysates from 100 μM urate- (versus vehicle-) treated astrocytes. (B) Urate induces protein products of Nrf2-targeted genes as shown by western blots of GCLM, GCLC and NQO-1 proteins in urate-treated astrocytes compared to controls. The approximate molecular weight of the indicated protein in kDa is indicated at Left. The graph represents densitometric analysis of the western blots to semi quantify the protein levels. (C) Quantitative PCR analysis of Nrf2 target genes in urate- (versus vehicle-) treated cells. Mean ± SEM are shown (n = 3). * denotes p value < 0.05.