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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Bone. 2015 Sep 28;81:620–631. doi: 10.1016/j.bone.2015.09.012

Fig. 3.

Fig. 3

Knee loading improved vessel remodeling in the femoral head. (A) The expression of VEGF was measured by immunohistochemistry assay. VEGF positive staining microvessels in femoral head were indicated by the red circles (200×, Bar = 100 μm). (B) The ratio of VEGF positively stained area to total field area was calculated. Osteonecrosis decreased the expression of VEGF, and knee loading enhanced them. (C) The density of microvessels was analyzed in the femoral head using ink blood perfusion angiography (100×, Bar = 200 μm). The representative photographs were shown. (D, E) Osteonecrosis decreased both the vessel number (D) and vessel volume (E), and knee loading significantly enhanced vessel number and volume. Asterisk (** and ***) represent statistical significance at p < 0.01 and p < 0.001, respectively (n = 6).