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. Author manuscript; available in PMC: 2015 Nov 10.
Published in final edited form as: Methods Enzymol. 2014;546:491–511. doi: 10.1016/B978-0-12-801185-0.00024-6

Figure 2.

Figure 2

The structure of Cas9 in complex with sgRNA and a single-stranded target DNA (ssDNA) adapted from Nishimasu et al. 2014 (PDB code 4OO8). The structure is shown in a surface representation and colored as in Fig. 1. Note, the inset is rotated 180° and displays the location of the sgRNA stem loops (SLs).