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. Author manuscript; available in PMC: 2015 Nov 10.
Published in final edited form as: Methods Enzymol. 2014;546:491–511. doi: 10.1016/B978-0-12-801185-0.00024-6

Figure 6.

Figure 6

Checking success of a screen and picking final clones. (A) An overview of primer design for the PDZ-dCas9 library. (B) Gel electrophoresis of PCRs run on the original PDZ-dCas9 library and the first and second round of screening. The banding patterns that appear after the first and second sorts are indicative of library enrichment, representing the insertion sites of a PDZ domain. It is also evident that the N and C termini fusions to PDZ are also enriched. Since these fusions are expected to be functional this serves as an internal control. (C) Fluorescent image of the on-plate 'finishing' screen. Colonies that express only GFP are expected to have a functional PDZ-dCas9.