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. Author manuscript; available in PMC: 2015 Nov 10.
Published in final edited form as: Methods Enzymol. 2014;546:491–511. doi: 10.1016/B978-0-12-801185-0.00024-6

Figure 7.

Figure 7

Validating functionality of engineered dCas9. (A) Sequence validation of the site 1188 PDZ intercalation. Sequence alignment via SnapGene. (B) Quantitative repression of GFP by the PDZ-1188 -dCas9 intercalation clone. Bulk florescence measurements of GFP expression levels over five hrs. Double asterisks represents a p value of < 0.0001 in a 1 way ANOVA. Single asterisk represents p values of < 0.0001 in an unpaired Student’s T-test. (C) Qualitative repression of ftsZ gene by PDZ-dCas9 and controls, scale bar is 5 µm.