Table 2.

Primers

Primer name	Length (BP)	Forward primer	Reverse primer
18 s rRNA for qPCR	149	5′ TCA ACT TTC GAT GGT AGT CGC CGT 3′	5′ TCC TTG GAT GTG GTA GCC GTT TCT 3′
β-actin mRNA for qPCR	149	5′ GCC GCC AGC TCA CCA TGG AT 3′	5′ CAC CAT CAC GCC CTG GTG CC 3′
PDK4 mRNA for qPCR	119	5′ CCT GTG AGA CTC GCC AAC A 3′	5′ TCC ACC AAA TCC ATC AGG CTC 3′
Cytochrome B genomic for qPCR	125	5′-TGA TAT TTC CTA TTC GCC TAC ACA-3′	5′-TGT TGT TTG GAT ATA TGG AGG ATG-3′
Pyruvate kinase genomic for qPCR	158	5′-GTC GAT CCA GGA GAA CAT ATC AT-3	5′-CTC CTA GTT TTC ACC CTC ATT TTC-3′
PDK4 genomic for sequencing	367	5′ CAC TGA GAA TGT GAC CCG CT 3′	5′ AGC CTT GTG TGA AGT AAC CTT AG 3′
PDK4 cDNA for sequencing	393	5′ ATC CTC CCG ACC CAA TTA GT 3′	5′ ACC CAC TGC TAC CAC ATC ACA 3′

Sequences of primers used to measure expression of human PDK4 mRNA, b-actin mRNA, 18s rRNA, pyruvate kinase from the nuclear genome and cytochrome B from the mitochondrial genom are shown along with sequencing primers for PDK4 using genomic DNA and cDNA templates