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. 2015 Nov 10;19:24. doi: 10.1186/s40824-015-0046-y

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© Subbiah et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Osteogenic induction of preosteoblasts on gelatin matrices. Immunofluorescence images of a, e type I collagen and b, f osteocalcin at day 7 and 14, respectively. c, g) Alizarin red and d, h von Kossa staining of preosteoblasts at day 7 and 14, respectively. Quantification of i alizarin red, j von kossa positively-stained area, k normalized ALP activity, and l calcium content, respectively. Results are mean ± SD of one triplicate approach that is representative of three independent experiments. *** p < 0.001 indicates a statistically significant difference