Table 3.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or characteristics | Source or reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | F− Ф80dlacZΔ (lacZYA-argF) U169 recA1 endA1 hsdR 17 (r −k m +k ) supE44 λ − thi-1 gyrA relA1 | Lab. strain |
| B. subtilis 168 | trpC2 | Lab. strain |
| B. subtilis WB800 | nprE aprE epr bpr mpr::ble nprB::bsr vpr wprA::hyg | [28] |
| Plasmids | ||
| pMA5ΔMCS | An intermediate plasmid excised from the original cloning site of pMA5 | [17] |
| p-Nsp-his | Encodes the unstructured protein Nsp | [17] |
| p-ssPhoD-Nsp-his | Encodes Nsp fused with signal peptide of PhoD | [17] |
| p-ssNprE-BgaB-his | Encodes BgaB fused with the signal peptide of NprE | This study |
| p-NCP-Nsp-hisa | Encodes four non-classically secreted protein-Nsp fusions | This study |
| p-NCP-PhoA-his | Encodes four non-classically secreted protein-PhoA fusions | This study |
| p-YvgN50-Nsp-his | Encodes the first 50 amino acids of YvgN fused with Nsp | This study |
| p-PhoA-his | Encodes intact Escherichia coli PhoA | This study |
| p-NCP-BgaB-his | Encodes four non-classically secreted protein-BgaB fusions | This study |
The four non-classically secreted proteins are GapA, PdhD, SodA and YvgN
aNCP represents the non-classically secreted proteins