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. 2015 Nov 10;15:275. doi: 10.1186/s12870-015-0660-9

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© Ramadan et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Thioester assay of 35 Arabidopsis E2s. The crude proteins for each of the 35 bio-E2s were incubated with FLAG-Ub for 5 min at 37 °C and treated with DTT or 8 M urea (-DTT). Immunoblot analysis against FLAG-Ub using anti-FLAG-HRP antibodies shows the presence of DTT-sensitive Ub conjugation activity for all E2s tested. Arrows show the expected E2-Ub adduct for each E2 in the absence of DTT. The side arrows show the free FLAG-Ub and expected FLAG-Ub adducts with WE2 and WE1 as arranged from bottom to top