Fig. 5.

Differentially expressed genes in PMA-induced gene differentiation in THP-1 cells stably expressing sPRDM16-WT and sPRDM16–K568R. a Functional annotation clustering (GO) of genes that were associated with SUMOylation of sPRDM16 in sPRDM16-WT and sPRDM16–K568R-THP-1 cells following incubation with PMA (3 nM/24 h; Analyzed by DAVID, grouped based on the biological process of level 1). b Differential gene expression between sPRDM16-WT and sPRDM16–K568R-THP-1 cells in mRNA-sequencing. c Expression of differentiation-related genes induced by PMA in THP-1 cells stably expressing sPRDM16-WT and sPRDM16–K568R (including control THP-1 cells with Lenti-Vector). KLF10, CCL5, IL6R, LIF, NUMB, BCL3 and HDAC9 was measured in Lenti-Vector, sPRDM16-WT and sPRDM16-K568R cells by Q-PCR. Data are presented as the △Ct between the gene of interest and the GAPDH levels expressed in the same sPRDM16-WT-transduced sample or sPRDM16-K568R-transduced sample, relative to the △Ct observed in the control sample. Mean from three independent experiments are depicted with S.D.