Fig. 7.
Dynamics between atypical PrPres and PrPSc in animals from the first and second passages. a Western blots of brain materials from animals of the first and second passages of F0.5-induced atypical PrPres. Brain material from an animal of the second passage of F0.5 (S05) included as a reference. b Western blots of brain materials from animals of the first, second and third passages of F0.5 fibrils. Arrows indicate animals that were used for preparing inoculums for the subsequent passages. Western blots were stained with SAF-84 or 3F4 antibody as indicated. c Brain materials from 263K- or S05-inoculated animals or second passage of F0.5 fibrils were treated with PNGase and PK, analyzed by Western blot and stained with 3F4