Fig. 3.

Correlation of pairwise distance of concatenated housekeeping genes with whole genome ANI values. a: Correlation of whole-genome ANI and pairwise distance of seven concatenated housekeeping genes of all 142 B. cereus sensu lato strains. Intra-cluster values (for clusters see Fig. 1) are depicted in black, all inter-cluster values in grey. Pearson’s correlation of all values is r² = 0.974. b and c: Intra-cluster comparison of whole-genome ANI and pairwise distance of seven concatenated housekeeping. Cluster affiliation (I – VII) according to species tree (Fig. 1). Strains that are difficult to sort into a distinct genomospecies are named and marked by circles. b: Clusters I, IV – VI can be distinguished at an ANI species boundary of ≥ 94 %. Cluster I: ANI 98.3 %, pairwise distance 0.005 substitutions per site. Cluster VII: ANI 99.5 %, pairwise distance 0.001 substitutions per site. c: Clusters II and III cannot be discerned on the basis of the comparisons presented: strain B. cereus F528/94 (#242) is affiliated to Cluster III by MLSA (Fig. 1) and whole-genome SNP analysis (Fig. 2), but to Cluster II by ANI (Additional file 2: Figure S1)