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. 2015 Nov 10;6:220. doi: 10.1186/s13287-015-0213-z

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© Xu et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Puromycin selection increases the percentage of cells expressing Sim1 in Sim1-Puro cultures. a–c Sim1 in situ hybridization (dark cell bodies) and nuclear staining (blue) on unselected cells. d–f Sim1 in situ hybridization and nuclear staining on selected cells. a & d Sim1 in situ hybridization. b & e Nuclear marker Hoechst (blue). c & f Overlaid images. g Selected cultures enriches for percentage of Sim1⁺ cells. * Denotes P <0.05 compared to unselected group