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. 2015 Nov 10;6:220. doi: 10.1186/s13287-015-0213-z

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© Xu et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Sim1-Puro cultures exhibit glutamatergic and neuronal markers through the first week post-selection. a–d Staining 3 days post-selection. e–h Staining 7 days post-selection. Immunocytochemistry marker labeled on far left. a & e β-III tubulin (red in combined); b & f VGluT2 (green in combined); c & g Combined image; d & h Zoomed in combined image. Scale bar = 100 μm