Table 1.

Primers for Sim1-Puro generation

Primer name	Sequence
pstartK_Sim1_upstream	AAAGTACTGTTTCTGGGGAAAACTCTAGTTTAGAGACCCTCCTGTTCTAAcgactgaattggttcctttaaagc
pstartK_Sim1_downstream	GAACCAGGCAGAGGGAAAGCTTCTCATTAGTGCTTTTCCCTTCTCTCTCCgccgcactcgagatatctagaccca
Sim1_CAT_Forward	GATGAGTCTGTGGAGTTTACGTTGTAAGAAGAAAGGGAGCCCGAGACACGGGCGCGCCagcattacacgtcttgagcgattgt
Sim1_CAT_Reverse 900 bp	TTGAGGAAGGGTGAGCAAATGGGAGATCAAAGAGCTCCTTCCCTGGAGAGGGCGCGCCcacttaacggctgacatgggaatta
Sim1_Fwd_Junction1	atgcacacgactcttcaaagaa
Puro_Reverse Junction1	gcgccaggaggccttccatctgttgct

Capital letters indicate homology arms for red recombination