Table 1. Primers Used and Their Characteristics.
Virus | Primer Sequence (5’ - 3’) | Genome Region Amplified | Amplicon, bp |
---|---|---|---|
VZV | ORF 8 | 275 | |
Forward | CACACGATAATGCCTGATCGG | ||
Reverse | TGCTGATATTTCCACGGTACAGC | ||
HSV-1 | RL 2 | 147 | |
Forward | TGGGACACATGCCTTCTTGG | ||
Reverse | CCCTTAGTCAGACTCTGTTACTTACCC | ||
HSV-2 | UL 28 | 227 | |
Forward | GTACAGACCTTCGGAGG | ||
Reverse | CGCTTCATCATGGGC | ||
EBV | Exon 4/5 terminal protein RNA | 182 | |
Forward | AACATTGGCAGCAGGTAAGC | ||
Reverse | ACTTACCAAGTGTCCATAGGAGC | ||
CMV | IRL 11 | 256 | |
Forward | GTACACGCACGCTGGTTACC | ||
Reverse | GTAGAAAGCCTCGACATCGC |