Table 1.

Nucleotide Sequences and the Purpose of Primers Used in This Study

primer	Sequence	purpose
For1	5′-GTAATTATATATGAATTGAATCTCC-3′	forward DNA sequencing
Rev1	5′-CTAATTGTTGTCTACGTTCAGAAAGATC-3′	reverse DNA sequencing
AOX1for	5′-GACTGGTTCCAATTGACAAGC-3′	external primer for sequencing
AOX1rev	5′-GCAAATGGCATTCTGACATCC-3′	external primer for sequencing
SapL3for	5′-AACTGCAGGCGTAATTATATATGAATTGAATC-3′	forward primer used for amplification with PstI extension
SapL3rev	5′-AGCGGCCGCATTGTTGTCTACGTTCAG-3′	reverse primer used for amplification with NotI extension
A14Cfor	5′-CTCCAAGGTACTACCAAGTGCCAATACTCGACATTTCTC-3′	mutagenesis
A14Crev	5′-GAGAAATGTCGAGTATTGGCACTTGGTAGTACCTTGGAG-3′	mutagenesis
S59Cfor	5′-GAGTTAATCTCAAAGCTTGCACTGGAACTGTCTCACTTG-3′	mutagenesis
S59Crev	5′-CAAGTGAGACAGTTCCAGTGCAAGCTTTGAGATTAACTC-3′	mutagenesis
A192Cfor	5′-GTACTTAACAATTTTGATACATGCAAGGAGGTTGAACCCGTTCC-3′	mutagenesis
A192Crev	5′-GGAACGGGTTCAACCTCCTTGCATGTATCAAAATTGTTAAGTAC-3′	mutagenesis
A246Cfor	5′-GGAGGGTGACTACTGTGTGCGAAGTGGAAATAGGGATC-3′	mutagenesis
A246Crev	5′-GATCCCTATTTCCACTTCGCACACAGTAGTCACCCTCC-3′	mutagenesis